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This Is A Custom Widget

This Sliding Bar can be switched on or off in theme options, and can take any widget you throw at it or even fill it with your custom HTML Code. Its perfect for grabbing the attention of your viewers. Choose between 1, 2, 3 or 4 columns, set the background color, widget divider color, activate transparency, a top border or fully disable it on desktop and mobile.

This Is A Custom Widget

This Sliding Bar can be switched on or off in theme options, and can take any widget you throw at it or even fill it with your custom HTML Code. Its perfect for grabbing the attention of your viewers. Choose between 1, 2, 3 or 4 columns, set the background color, widget divider color, activate transparency, a top border or fully disable it on desktop and mobile.

This Is A Custom Widget

This Sliding Bar can be switched on or off in theme options, and can take any widget you throw at it or even fill it with your custom HTML Code. Its perfect for grabbing the attention of your viewers. Choose between 1, 2, 3 or 4 columns, set the background color, widget divider color, activate transparency, a top border or fully disable it on desktop and mobile.

Identification & Characterization

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Identification & Characterization 2017-09-20T14:58:51+00:00

CARD’s platform facilitates the automated selection of aptamers, performing twelve selection cycles without manual interference. We developed a selection protocol that facilitates the identification of RNA aptamers binding to protein targets. This protocol enables the identification of enriched libraries within a short timeframe. Figure 1 represent the individual steps of one selection cycle: i) the incubation of RNA libraries with the protein target , ii) separation of bound from unbound nucleic acids, iii) recovery of bound nucleic acids, iv) RT-PCR amplification, and v) in vitro transcription. However, protocols for covering other targets, e.g. cells or small molecules will be implemented and available soon.

 

Figure 1: Flowchart highlighting the consecutive modules of one selection cycle for the identification of RNA aptamers.

Besides the standard automated selection, we also offer an optional support as a post-selection phase, which includes Next Generation Sequencing (NGS), as well as affinity and specificity determination of the resultant aptamers.